讲座题目:Resolving Trk signaling through proteomics

主讲人:Ralph A.Bradshaw 教授

主持人:袁崇刚 教授

开始时间:2014-06-23 11:00

讲座地址:闵行校区bevictor伟德官网534报告厅

主办单位:bevictor伟德官网 科技处

 

报告人简介：

Ralph A. Bradshaw is Professor Emeritus, Physiology & Biophysics –School of Medicine, UC Irvine and Professor,Department of Pharmaceutical Chemistry, University of California, San Francisco. He obtained his PhD from Duke University and was a postdoctoral fellow at Indiana University (Chemistry) and University of Washington, Seattle (Biochemistry). He was a member of the Washington University (St.Louis), School of Medicine faculty from 1969 to 1982 and the UCI faculty from 1982 to 2006. His research interests are on molecular basis of signal transduction by growth factors, post-translational modifications andprotein N-terminal processsing.

 

报告内容摘要：

Only two established sites (Y490 and Y785) on the NGF receptor (TrkA) endodomain are known to be directly involved in signal propagation following activation by ligand. We utilized PC12 cell lines, stably transfected with chimeric receptors, to map the TrkA downstream phosphoproteome determined  by LC MS/MS  after 20 mins of stimulation  of the ‘native’ receptor and mutants with Y490F and  Y490/785F.The proteins identified that were significantly modified on serine or  TrkA signalling. In contrast the mutant samples showed entities, which threonine in the ‘native’ sample contained many of the well-established participants in were either dependent or not on these docking site(s),and a clear subset that was not dependent on either, confirming that there are as yet unidentified sites on TrkA that are involved in downstream signaling.